Purification and properties of thermostable xylanase and beta-xylosidase produced by a newly isolated Bacillus stearothermophilus strain
نویسندگان
چکیده
منابع مشابه
Purification and characterization of an acidic, thermophilic phytase from a newly isolated Geobacillus stearothermophilus strain DM12
Microbial phytases were applied mainly to animal and human foodstuffs in order to improvemineral bioavailability and food processing. In addition, phytases have potentialbiotechnological application in various other fields, such as environmental protection,aquaculture and agriculture. Bacillus sp. DM12, an isolate from a hot spring, produces phytase,which catalyzes the hydrolysis of phytic acid...
متن کاملPurification and Some Properties of a Thermostable Protease, BSP2, Produced from Bacillus stearothermophilus No. 2
A thermophilic bacteria, Uac3l~n.s stl!rtrothe?-rr~oplr iltts No. 2 isolated from sewage sludge compost, was found to produce a thermostable neutral protease (BSP2). The BSP2 was purified from culture broth by fractionation with cold acetone and subsequent column chromatographies. The purified BSP2 gave a single band on SDS PAGE and its molecular weight was estimated to be 35kDa. The purified p...
متن کاملOverexpression and single-step purification of a thermostable xylanase from Bacillus stearothermophilus T-6.
Xylanase T-6 is a thermostable alkaline-tolerant enzyme that is produced by Bacillus stearothermophilus T-6. Xylanase T-6 was found to bleach pulp effectively at pH 9 and 65 degrees C and was used successfully on an industrial-scale mill trial. To facilitate the future characterization of the protein via X-ray analysis and protein engineering, it was necessary to overexpress the enzyme in Esche...
متن کاملIsolation and Partial Characterization of a Bacterial Thermostable Polymethyl Galacturonase from a Newly Isolated Bacillus sp. strain BR1390
Background: Pectinases are pectin degrading class of enzymes including polygalacturonase (PG), polymethyl galacturonase (PMG), pectate lyase (PEL), and pectin esterase (PE) that are commonly used in processes involving the degradation of plant materials, such as speeding up the extraction of fruit juices. Objectives: A highly methylated pectin degrading bacterium from soil covered with fruit wa...
متن کاملpurification and characterization of an acidic, thermophilic phytase from a newly isolated geobacillus stearothermophilus strain dm12
microbial phytases were applied mainly to animal and human foodstuffs in order to improvemineral bioavailability and food processing. in addition, phytases have potentialbiotechnological application in various other fields, such as environmental protection,aquaculture and agriculture. bacillus sp. dm12, an isolate from a hot spring, produces phytase,which catalyzes the hydrolysis of phytic acid...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1990
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.172.12.6669-6672.1990